The second monoclonal antibodies had been ordered of Pharmingen (Hillcrest, Calif Cellphone staining and move cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaNstep 3) with optimal amounts…